Appendix D — Map Validation

These figures present validation of my 3D models. In each, panel A is a local resolution estimation for the α, β, and γ subunits moving from left to right. The color scales for this panel vary slightly in an attempt to capture the resolution range of each map. Panel B is a particle orientation distribution plot. Note that in this panel the color scheme is logarithmic. Panel C is a global and 3DFSC plot. The global FSC is plotted in black, while the histogram of directional FSCs are plotted as a grey histogram. The exact values of the histogram are presented below the histogram as black marks, jittered slightly to give a sense of overlap. Finally, panel D is the model-map FSC.

Figure D.1: Validation of human CKO map and model.

Figure D.2: Validation of human CKO-DEG map and model.

Figure D.3: Validation of human CKO-DEG monofab map and model.

Figure D.4: Validation of human CKO nanodisc map and model.

Figure D.5: Validation of mouse uncleaved map and model.

Figure D.6: Validation of trypsin-cleaved mouse map and model.